Coding
Part:BBa_K2315100:Design
Designed by: MingZhe Chen Group: iGEM17_Shanghaitech (2017-10-26)
lasI with 6*his tag and double TAA
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 223
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
To make sure the existence of lasI, we add 6*his tag between double TAA, which is designed to decrease the leaky of the expression of lasI
Source
DNA synthesize from commercial company