Coding

Part:BBa_K2315100:Design

Designed by: MingZhe Chen   Group: iGEM17_Shanghaitech   (2017-10-26)


lasI with 6*his tag and double TAA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 223
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

To make sure the existence of lasI, we add 6*his tag between double TAA, which is designed to decrease the leaky of the expression of lasI


Source

DNA synthesize from commercial company

References